Agenda > DRomics tool training course

DRomics tool training course

Management of molecular data obtained in a dose-response framework

Omics methods are increasingly used in ecotoxicology to explore stressor effects at the molecular scale, to investigate molecular pathway responses or to discover new biomarkers. Applied in a dose-response framework, they open new perspectives for mechanistic understanding (e.g. adverse-outcome pathway concept) and risk assessment but the specificity and the complexity (e.g. non-monotonic) of their responses require a dedicated workflow to manage properly the data. For that purpose, the DRomics tool (Larras et al., 2018) was recently developed in order to :

• detect the responding molecular items (e.g. metabolites, transcripts),
• find out the best model describing their response,
• build the dose-response curve and
• obtain an effect concentration.

The objectives of this training course are:

• to learn how to use the DRomics tool (online app)
• understand the rationale behind each step of the workflow in order to optimize the parameter setting
• Explore the outputs of the tool and exchange about their use in downstream analysis

No skills in R are mandatory.

We just ask the participants to bring their own laptop. Test datasets will be provided but the participants are also welcomed to come with their own data.

 Reference: Larras F., Billoir E., Baillard V., Siberchicot A., Scholz S., Wubet T., Tarkka M., Schmitt-Jansen M., Delignette-Muller ML. DRomics: A Turnkey Tool to Support the Use of the Dose–Response Framework for Omics Data in Ecological Risk Assessment. Environmental Science & Technology 52, 14461–14468 (2018).